Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, PE-Alexa Fluor 647

货号：A-20990

规格：100 µg

价格：5153

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：其它

抗体类型:	荧光二抗	同型对照：
浓度：	1 mg/mL	用法：	1-10µg/mL (Flow)

靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据：

Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A-20990) in Flow Simultaneous detection of three cell surface markers using an Alexa Fluor® 647-R-phycoerythrin tandem conjugate, Alexa Fluor® 488 dye and R-phycoerythrin labels. Lymphocytes from ammonium chloride RBC-lysed whole blood were labeled with a mouse anti-human CD3 monoclonal antibody, washed with 1% BSA in PBS and then incubated with a goat anti-mouse IgG antibody labeled with the Alexa Fluor® 647-R-phycoerythrin tandem dye (Product # A-20990). Cells were again washed and then labeled with directly conjugated primary antibodies against the CD8 and CD4 markers (Alexa Fluor® 488 dye-labeled mouse anti-human CD8 antibody (Product # A21339) and R-phycoerythrin-conjugated mouse anti-human CD4 antibody (Product # A21337). After a further wash in 1% BSA/PBS, labeling was analyzed on a Becton Dickinson FACScan flow cytometer using excitation at 488 nm. CD8 was detected in the green channel (525 ± 10 nm), CD4 in the orange channel (575 ± 10 nm) and CD3 in the red channel (>650 nm). The bivariate scatter plots show the expected mutually exclusive populations of CD4 and CD8 positive cells (panel A), together with co-positive CD3/CD4 (panel B) and CD3/CD8 (panel C) populations.

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参考文献：

1.Scientific reportsThe E3 ligase Mule protects the heart against oxidative stress and mitochondrial dysfunction through Myc-dependent inactivation of Pgc-1α and Pink1.2. AutophagySNAPIN is critical for lysosomal acidification and autophagosome maturation in macrophages.3. GlycobiologyFishing for lectins from diverse sequence libraries by yeast surface display - an exploratory study.4.Journal of immunology (Baltimore, Md. : 1950)Disparate primary and secondary allospecific CD8+ T cell cytolytic effector function in the presence or absence of host CD4+ T cells.

技术参数

产品应用 Flow;IHC;ICC;IHC(F);Misc

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