Goat anti-Mouse IgG Fc Secondary Antibody, HRP/山羊抗小鼠IgG Fc二抗，HRP

货号：31437

规格：2 mL

价格：4081

产品类型：荧光二抗

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG

荧光染料：HRP

抗体类型:	荧光二抗	同型对照：
浓度：	0.8mg/mL	用法：	1:500-1:5,000 (ICC);1:500-1:5,000 (IHC);1:10,000-1:200,000 (WB)

产品详细信息Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.Product # 31437 has been successfully used in Western blot, and ICC applications.Antibody Specificity: This antibody reacts with the heavy chains of mouse IgG, but not with the light chains common to most mouse immunoglobulins, based on immunoelectrophoresis. No antibody was detected against non-immunoglobulin serum proteins or against mouse IgM. However, this antibody may cross-react with immunoglobulins from other species.Restoration and Storage: Store product at 4°C until opened. Restore with 2.0 mL distilled water (0.8 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day.To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.Country of Origin: USA 靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据：

Mouse IgG Fc Secondary Antibody (31437) in WB Western blot analysis of Vascular Endothelial Growth Factor (VEGF) was performed by loading 1 µg of indicated recombinant VEGF isoforms, and 10 µL of PageRuler PlusPrestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane (Product # 88518) using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk/TBST for at least 1 hour at room temperature. VEGF was detected at 14 kD, 19 kD and 22-30 kD using a VEGF monoclonal antibody (Product # MA5-12184) at a concentration of 1 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody (Product # 31437) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34076).

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参考文献：

1.Neoplasia (New York, N.Y.)Nidogen-1 Degraded by Cathepsin S can be Quantified in Serum and is Associated with Non-Small Cell Lung Cancer.2.Scientific reportsNovel role of cortactin in G protein-coupled receptor agonist-induced nuclear export and degradation of p21Cip1.3.Human gene therapyHuman, Pig, and Mouse Interferon-Induced Transmembrane Proteins Partially Restrict Pseudotyped Lentiviral Vectors.

技术参数

产品应用 ICC;IHC;WB;ELISA

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