Rabbit anti-Mouse IgG2b Recombinant Secondary Antibody/大鼠抗小鼠IgG2b重组二抗

货号：SA5-10210

规格：100 µg

价格：2732

产品类型：二抗

品牌：Thermo Fisher

抗原：Peptide corresponding to Mouse IgG2b.

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：RM108

荧光染料：其它

抗体类型:	二抗	同型对照：
浓度：	1 mg/mL	用法：	0.005-0.2 µg/mL (ELISA)； 0.5-2 µg/mL (ICC)； 0.5-2 µg/mL (IF); 0.5-2 µg/mL (IHC); 0.1-0.5 µg/mL (WB)

产品详细信息This antibody reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. 靶标信息IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

数据：

Mouse IgG2b Secondary Antibody (SA5-10210) in WB Western blot of nonreduced (-) and reduced (+) mouse IgG2b (20 ng/lane), using 0.2 µg/mL of Rabbit Anti-Mouse IgG2b Secondary Antibody (Product # SA5-10210). This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced g2b form (~50 kDa). Mouse IgG2b Secondary Antibody (SA5-10210) in ELISA ELISA of mouse immunoglobulins shows Rabbit Anti-Mouse IgG2b Secondary Antibody (Product # SA5-10210) reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of Rabbit Anti-Mouse IgG2b Secondary Antibody (Product # SA5-10210) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. Mouse IgG2b Secondary Antibody (SA5-10210) in ELISAA titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of Rabbit Anti-Mouse IgG2b Secondary Antibody (Product # SA5-10210) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

技术参数

产品应用 ELISA;ICC;IF;IHC;WB

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-21884.htm