Rabbit anti-Mouse IgG2a (Kappa light chain) Recombinant Secondary Antibody/兔抗小鼠Ig2a（κ轻链）重组二抗

货号：SA5-10219

规格：100 µg

价格：2732

产品类型：二抗

品牌：Thermo Fisher

抗原：Peptide corresponding to Mouse IgG.

物种：小鼠

宿主：兔

抗体亚型：IgG

克隆号：RM107

荧光染料：其它

抗体类型:	二抗	同型对照：
浓度：	1 mg/mL	用法：	0.005-0.2 µg/mL (ELSIA)； 0.5-2 µg/mL (ICC)； 0.5-2 µg/mL (IHC); 0.5-2 µg/mL (IF); 0.1-0.5 µg/mL (WB)

产品详细信息This antibody reacts to the Fab region of mouse IgG2ak. No cross reactivity with mouse IgG2al, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. 靶标信息IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

数据：

Mouse IgG2a (Kappa light chain) Secondary Antibody (SA5-10219) in WBWestern blot of nonreduced (-) and reduced (+) mouse IgG2ak (20 ng/lane), using 0.2 µg/mL of Rabbit Anti-Mouse IgG2a kappa Secondary Antibody (Product # SA5-10219). This antibody only reacts to nonreduced Mouse IgG2ak. Mouse IgG2a (Kappa light chain) Secondary Antibody (SA5-10219) in ELISA ELISA of mouse immunoglobulins shows RM107 reacts to the Fab region of mouse IgG2ak; no cross reactivity with IgG2al, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of Rabbit Anti-Mouse IgG2a kappa Secondary Antibody (Product # SA5-10219) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. Mouse IgG2a (Kappa light chain) Secondary Antibody (SA5-10219) in ELISA A titer ELISA of mouse IgG2ak. The plate was coated with different amounts of mouse IgG2ak. A serial dilution of Rabbit Anti-Mouse IgG2a kappa Secondary Antibody (Product # SA5-10219) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

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技术参数

产品应用 ELISA;ICC;IHC;IF;WB

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