Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP/山羊抗小鼠IgG (H+L)高交叉吸附二抗，HRP

货号：A16078 ,A16078SAMPLE

规格：1 mg,50 μg

价格：2151,440

产品类型：二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：HRP

抗体类型:	荧光二抗	同型对照：
浓度：		用法：	1:500-1:20,000 (ELISA)； 1:500-1:20,000 (IHC); 1:10,000-1:200,000 (WB)

靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A16078) in WB Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and U-87 MG (Lane 2). The blots were probed with Anti-SOD2 Mouse Monoclonal Antibody (Product # MA1-106, 0.25 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP (Product # A16078) at dilutions 1:10,000 (Fig. 1), 1:100,000 (Fig. 2) and 1:200,000 (Fig. 3). A 25 kDa band corresponding to SOD2 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A16078) in WBWestern blot analysis of CXCR1/IL8RA was performed by loading 20 µg of indicated whole cell lysates in reducing sample buffer and 8 µL PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BOX). Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk/TBST for at least 1 hour at room temperature. CXCR1/IL8RA was detected using a CXCR1/IL8RA mouse monoclonal antibody (Product # MA1-206) at a concentration of 1 µg/mL in blocking buffer overnight at 4°C on a rocking platform, followed by a HRP conjugated secondary antibody (Product # A16078) at a dilution of 1:5000 for at least 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Dura Extended Duration Substrate (Product # 34076) and the myECL Imager (Product # 62236).

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参考文献：

1. PsychoneuroendocrinologyMaternal care and affective behavior in female offspring: Implication of the neurosteroid/GABAergic system.

技术参数

产品应用 ELISA;IHC;WB

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