Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, DSB-X Biotin/山羊抗小鼠IgG (H+L)高交叉吸附二抗，DSB-X生物素

货号：D-20691

规格：1 mg

价格：2577

产品类型：二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Biotin

抗体类型:	荧光二抗	同型对照：
浓度：	2 mg/mL	用法：	1-10 µg/mL(ICC);1-10 µg/mL（IF)

产品详细信息DSB-X biotin is a derivative of desthiobiotin, a stable biotin percursuor. DSB-X biotin utilizes a seven-atom spacer to increase the available of the DSB-X biotin conjugate to bind in the deep biotin-binding pocket of streptpavidin or avidin. Whereas harsh chaotropic agents and low pH are required to dissociate a biotin complex from avidin or steptavidin, binding can be reversed by adding excess D-desthiobiotin or natural D-biotin at neutral pH and at room temperature. 靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据：

Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (D-20691) in IFReversible binding by DSB-X™ biotin. Microtubules of fixed bovine pulmonary artery endothelial cells were labeled with mouse monoclonal anti-a-tubulin antibody (Product # A11126), detected with either biotin-XX goat anti-mouse IgG antibody (Product # B-2763, panel A) or DSB-X™ biotin goat anti-mouse IgG antibody (Product # D-20691, panel B) and visualized with green-fluorescent Alexa Fluor® 488 streptavidin (Product # S-11223). Nuclei were stained with blue-fluorescent DAPI (Product # D1306, D3571, D21490). After incubating with 10 mM d-biotin (Product # B-1595, B20656), the binding between the biotinylated antibody is unaltered (panel C), whereas the streptavidin conjugate has been stripped from the DSB-X™ biotin-labeled antibody (panel D).

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参考文献：

1.The ProstateKeratinocyte-derived chemokine induces prostate epithelial hyperplasia and reactive stroma in a novel transgenic mouse model. 2.Analytical biochemistry Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation.

技术参数

产品应用 ICC;IF;IHC;Misc

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