F(ab')2-Rabbit anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, FITC/F(ab')2-兔抗小鼠 IgG (H+L) 交叉吸附二抗, FITC
货号：PA1-84348
规格：1mg
价格：5984
产品类型：荧光二抗
品牌：Thermo Fisher
抗原：Purified murine IgG.
物种：小鼠
宿主：兔
抗体亚型：IgG
荧光染料：FITC
| 类型: | 单抗 | 同型对照： | |
| 浓度： | 1 mg/mL | 用法： | 1:25-1:100(Flow) |
产品详细信息Some cross reactivity with mouse IgM and IgA is expected, as is cross reactivity with rat IgG. Cross reactivity with human serum proteins has been minimized by solid phase adsorption. F(ab')2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove the remaining intact IgG or Fc fragments.For Flow Cytometry, use 50 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.FITC conjugated Rabbit F(ab')2 anti Mouse IgG antibody recognizes all subclasses of mouse IgG.靶标信息IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
QQ:1749072012