CD25 Monoclonal Antibody (CD25-4E3), APC, eBioscience™/CD25单抗(CD25-4E3), APC, eBioscience™

货号： 17-0257-42, 17-0257-41

规格：100 tests,25 tests

价格：2925,1151

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：CD25-4E3

荧光染料：APC

类型:	单抗	同型对照：	Mouse IgG2b kappa Isotype Control (eBMG2b), APC, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.06 µg)/test(Flow)

产品详细信息Description: This CD25-4E3 monoclonal antibody reacts to human CD25, which is also known as the low-affinity interleukin (IL)-2 receptor alpha. CD25 is expressed on activated T cells (including a subset of regulatory T cells), B cells, and macrophages. This receptor subunit associates with CD122, the IL-2 receptor beta chain, and CD132, the common gamma chain, to form the high-affinity IL-2 receptor. IL-2 receptor signaling has been linked to cell proliferation and survival in lymphocytes. CD25is a marker for Hodgkin's disease, and has been associated with such autoimmune diseases as Type I diabetes.This monoclonal antibody is cross-reactive to rhesus monkey.Applications Reported: This CD25-4E3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This CD25-4E3 antibody has been pre-titrated and tested by flow cytometric analysis of nrmal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

数据

图1 CD25 Antibody (17-0257-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD4 FITC (Product # 11-0048-42) and Mouse IgG2b K Isotype Control APC (Product # 17-4732-81) (left) or Anti-Human/Non-Human Primate CD25 APC (right). Cells in the lymphocyte gate were used for analysis. 图2 Staining of stimulated human peripheral blood cells. As expected based on known expression patterns, CD25 clone CD25-4E3 staining is significantly increased in CD4+ T cells after stimulation. Details: Normal human peripheral blood cells were unstimulated (blue histogram) or stimulated for 3 days with Concanavalin A (Con A) Solution (500X) and Lipopolysaccharide (LPS) Solution (500X) (purple histogram). Cells were stained with Fixable Viability Dye eFluor 780, CD4 (clone SK3) and CD25 (clone CD25-4E3). Viable cells in the CD4+ gate were used for analysis.

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参考文献：

1.OncotargetHDAC inhibition potentiates immunotherapy in triple negative breast cancer.2.Cancer immunology, immunotherapy : CIITumor specific regulatory T cells in the bone marrow of breast cancer patients selectively upregulate the emigration receptor S1P1.3.OncoimmunologyHLA Class II tetramers reveal tissue-specific regulatory T cells that suppress T-cell responses in breast carcinoma patients.

技术参数

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