FGF2 (bFGF) Mouse ELISA Kit/小鼠FGF2（bFGF）ELISA试剂盒
货号：EMFGF2,EMFGF2X5,EMFGF2X10
规格：96 tests,5X96 tests,10X96 tests
价格：7311,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 8 pg/mL |
| 检测范围 | 18.8-600 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 样品稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | FGF2 |
| Protein aliases | Basic fibroblast growth factor, bFGF, FGF-2, Heparin-binding growth factor 2, HBGF-2, Fibroblast growth factor 2 |
物种（Tested） | 小鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | bFGF, Fgf-2, Fgf2, Fgfb |
| Gene ID | (Mouse) 14173 |
| Gene symbol | Fgf2 |
| UniProt ID | (Mouse) P15655 |
The Mouse FGF2 (bFGF) ELISA quantitates Ms FGF2 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms FGF2.小鼠FGF2（bFGF）ELISA定量检测小鼠血清，血浆或细胞培养基中的Ms FGFs。 该检测试剂盒将专门识别天然和重组Ms FGF2。Principle of the method 方法原理The Mouse FGF2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠FGF2固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.FGF2（FGFb，碱性成纤维细胞生长因子）属于成纤维细胞生长因子（FGF）家族，并与高亲和力跨膜受体相互作用，影响细胞增殖和组织新血管形成。 FGF2以具有不同细胞内定位和功能的五种同工型形式存在。 18 kDa的同工型主要是胞质的，并通过细胞表面受体起作用，而22、22.5、24和34 kDa的同工型是有核的，并且可能独立于跨膜受体途径发出信号。在人类中，该基因位于4号染色体的q臂上。FGF2与多种生物学过程有关，例如肢体和神经系统发育，伤口愈合和肿瘤生长。 FGF2的mRNA包含多个聚腺苷酸化位点，并交替从非AUG和AUG起始密码子翻译，产生具有不同特性的五个不同同工型。 CUG起始的同工型位于细胞核中，负责内分泌作用，而AUG起始的形式主要是胞质的，并负责该FGF的旁分泌和自分泌作用。与FGF2功能障碍相关的疾病包括卡波济肉瘤和角膜新生血管。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
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