Mouse IgG2b kappa Isotype Control (eBMG2b), PerCP-eFluor 710
货号：46-4732-80,46-4732-82
规格：25μg,100μg
价格：1029,2365
产品类型：小鼠同型对照
品牌：eBioscience
物种：其它
宿主：小鼠
抗体亚型：IgG2b, kappa
克隆号：eBMG2b
荧光染料：PerCP-eFluor™ 710
| 类型: | Isotype Control | 同型对照： | |
| 浓度： | 0.2 mg/mL | 用法： | Assay-Dependent (Ctrl)；Assay-Dependent(Flow) |
产品详细信息Description: The monoclonal mouse IgG2b, kappa is useful as an isotype control immunoglobulin.Applications Reported: Mouse IgG2b Isotype Control PerCP-eFluor® 710 has been reported for use in flow cytometric analysis.Applications Tested: Mouse IgG2b K Isotype Control PerCP-eFluor® 710 has been tested by flow ctometric analysis of normal human peripheral blood cells. Use isotype control at the same concentration as experimental antibody.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
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