TNF beta Monoclonal Antibody (359-81-11), APC
货号：17-7327-81
规格：50μg
价格：1649
产品类型：流式抗体
品牌：eBioscience
物种：人
宿主：小鼠
抗体亚型：IgG
克隆号：359-81-11
荧光染料：APC
| 类型: | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC |
| 浓度： | 0.2 mg/mL | 用法： | 0.125 µg/test(Flow) |
产品详细信息Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.Applications Reported: APC Mouse IgG1 K Isotype Control has been reported for use in flow cytometric analysis.Applications Tested: The Mouse IgG1 K Isotype Control can be used at the same concentration as the experimental antibody.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
QQ:1749072012