Rat IgG1 kappa Isotype Control (eBRG1), Biotin

货号：13-4301-81,13-4301-82,13-4301-85

规格：50μg,100μg,500μg

价格：0,1132,2788

产品类型：大鼠同型对照

品牌：eBioscience

物种：其它

宿主：大鼠

抗体亚型：IgG

克隆号：eBRG1

荧光染料：Biotin

类型:	Isotype Control	同型对照：
浓度：	0.5 mg/mL	用法：	Assay-Dependent(Ctrl)；Assay-Dependent(Flow)；Assay-Dependent(ICC)；Assay-Dependent (IF)；Assay-Dependent(IHC)

产品详细信息Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG1 isotype control can be used in immunohistochemistry, immunocytochemistry, flow cytometric analysis, and ELISA.Applications Tested: This rat IgG1 isotype control has been used by flow cytometric analysis of mouse splenocyte suspension and can be used at the same concentration as the experimental antibody.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

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参考文献：

1.SpringerPlusThe Ser/Thr kinase MAP4K4 drives c-Met-induced motility and invasiveness in a cell-based model of SHH medulloblastoma."13-4301 was used as a Control in experiments to investigate whether HGF could drive dissemination of medulloblastoma cells expressing high levels of c-Met."AuthorsSanthana Kumar K,Tripolitsioti D,Ma M,Grählert J,Egli KB,Fiaschetti G,Shalaby T,Grotzer MA,Baumgartner M2.Stem cells and developmentIn vitro multilineage differentiation and self-renewal of single pancreatic colony-forming cells from adult C57BL/6 mice."13-4301 was used in Flow cytometry/Cell sorting to show that the pancreatic colony assays allow quantitative analyses of progenitors at a single-cell level, useful for elucidating in vitro mechanisms."AuthorsJin L,Feng T,Zerda R,Chen CC,Riggs AD,Ku HT

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