IL-13 Monoclonal Antibody (eBio13A), PE

货号：12-7133-41

规格：25 Tests

价格：1074

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：eBio13A

荧光染料：PE

类型:	单抗	同型对照：
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test(Flow)

产品详细信息Description: The eBio13A antibody reacts with mouse IL-13. IL-13 is a cytokine produced mainly by Th2 cells, but also by antigen-primed CD8 T cells. IL-13 has a strong involvement in allergic inflammation and parasitic clearing and in cancer models has been shown to have either inhibitory or stimulatory activity depending on the tumor. In humans, IL-13 is found to play a role in isotype switching in B cells. IL-13 is implicating in down modulating macrophage activity, through the reduction of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12)Applications Reported: The eBio13A antibody has been reported useful for ELISA and ELISPOT capture, as well as intracellular staining for flow cytometric analysis.Applications Tested: This eBio13A antibody is tested intracellular staining of cultured mouse splenocytes. This product has been pre-titrated and tested intracellular staining of cultured mouse splenocytes. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

数据

IL-13 Antibody (12-7133-41)Genome medicine 2018 -Fig. 6 IRE1a-XBP1 pathway is required for cytokine expression and secretion in Th2 lymphocyte. Naive T helper cells were cultured following Th2 activation condition in the presence of IRE1a inhibitor 4mu8c for 3 days, rested for 2 days, reactivated by coated plate, and analyzed by flow cytometry to detect intra-cellular cytokines IL4, IL5, and IL13 expression. Representative FACS profiles are displayed in the first two columns. The intra-cellular cytokine expression is compared in column 3, with three to seven independent biological replicates. Fourth column: cell culture supernatants from 4mu8c-treated or DMSO-treated Th2 were analyzed by ELISA to measure the cytokine concentration. FACS gating: lymphocytes > singlets > live cells > cytokines

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参考文献：

1.Genome medicineGenome-wide analyses reveal the IRE1a-XBP1 pathway promotes T helper cell differentiation by resolving secretory stress and accelerating proliferation."Published figure using IL-13 monoclonal antibody (Product # 12-7133-41) in Flow Cytometry"2.ImmunityAutoimmune Th17 Cells Induced Synovial Stromal and Innate Lymphoid Cell Secretion of the Cytokine GM-CSF to Initiate and Augment Autoimmune Arthritis."Published figure using IL-13 monoclonal antibody (Product # 12-7133-41) in Flow Cytometry"3.Arthritis research and therapyFli1-haploinsufficient dermal fibroblasts promote skin-localized transdifferentiation of Th2-like regulatory T cells."Published figure using IL-13 monoclonal antibody (Product # 12-7133-41) in Flow Cytometry"4.ImmunityHpARI Protein Secreted by a Helminth Parasite Suppresses Interleukin-33."Published figure using IL-13 monoclonal antibody (Product # 12-7133-41) in Flow Cytometry"5.ImmunityEpidermal Growth Factor Receptor Expression Licenses Type-2 Helper T Cells to Function in a T Cell Receptor-Independent Fashion."Published figure using IL-13 monoclonal antibody (Product # 12-7133-41) in Flow Cytometry"

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