CD36 Monoclonal Antibody (eBioNL07 (NL07)), FITC

货号：11-0369-41,11-0369-42

规格：25 Tests,100 Tests

价格：1029,2055

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgM

克隆号：eBioNL07 (NL07)

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgM Isotype Control (11E10), FITC
浓度：	5 µL/Test	用法：	5 μL (0.25 μg)/test(Flow)

产品详细信息Description: The monoclonal antibody eBioNL07 recognizes human CD36, which is a member of the class B scavenger receptor family. CD36 was originally identified as a platelet-membrane glycoprotein also called glycoprotein IV and a receptor for thrombospondin-1 (TSP-1) and extracellular matrix proteins. Binding to TSP-1 is in the CLESH (CD36 LIMP-II Emp sequence homology) domain of CD36. CD36 expression is broad and includes microvascular (but not large vessel) endothelium, adipocytes, skeletal muscle, dendritic cells, epithelia of the retina, breast, and intestine, smooth muscle cells, and hematopoietic cells, including erythroid precursors, platelets, monocytes/macrophages, DCs and megakaryocytes. Expression on platelets is absent on Nak-a negative donors. Unlike other scavenger receptor, CD36 binds LDL that has been exposed to "minimally" oxidizing conditions. CD36 is also a fatty acid translocase (FAT) necessary for the transport of long-chain fatty acids (LCFAs) and therefore may play a role in atherosclerosis.Applications Reported: This eBioNL07 (NL07) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioNL07 (NL07) antibody has been pre-titrated and tested by flow cytometric analysis. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD36 (fatty acid translocase, FAT) is an 88 kDa, ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and is rapidly induced on Follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similar to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids. CD36 is preferentially found within lipid rafts, which facilitates its association with receptors, signaling and adapter molecules. CD36 binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. Further, CD36 may function as a cell adhesion molecule and directly mediates cyto-adherence of Plasmodium falciparum parasitized erythrocytes. Mutations in the CD36 gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants encoding the CD36 protein have been found.

数据

CD36 Antibody (11-0369-41) in FlowStaining of normal human peripheral blood cells with Mouse IgM Isotype Control FITC (Product # 11-4752-80) (blue histogram) or Anti-Human CD36 FITC (purple histogram). Cells in the monocyte gate were used for analysis.

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参考文献：

1.The Journal of investigative dermatologySpatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations."Published figure using CD36 monoclonal antibody (Product # 11-0369-42) in Flow Cytometry"2.ImmunologyInterferon-γ enhances both the anti-bacterial and the pro-inflammatory response of human mast cells to Staphylococcus aureus."11-0369 was used in Flow cytometry/Cell sorting to investigate the effect of interferon-γ on the biological responses of human mast cells to Staphylococcus aureus."AuthorsSwindle EJ,Brown JM,Rådinger M,DeLeo FR,Metcalfe DD3.Human molecular geneticsDevelopmental transcriptome analysis of human erythropoiesis."11-0369 was used in Flow cytometry/Cell sorting to perform RNA-seq on CD34(+) cells after ex vivo differentiation from the earliest into the most mature erythroid cell stages."AuthorsShi L,Lin YH,Sierant MC,Zhu F,Cui S,Guan Y,Sartor MA,Tanabe O,Lim KC,Engel JD4.Scientific reportsIRGM1 regulates oxidized LDL uptake by macrophage via actin-dependent receptor internalization during atherosclerosis."Published figure using CD36 monoclonal antibody (Product # 11-0369-42) in Flow Cytometry"5.OncogeneTerminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming."11-0369 was used in Flow cytometry/Cell sorting to investigate the potential of nuclear reprogramming as a broadly applicable therapeutic strategy for the treatment of cancer."

技术参数

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