CD40 Monoclonal Antibody (HM40-3), FITC

货号：11-0402-81,11-0402-82,11-0402-85,11-0402-86

规格：50μg,100μg,500μg,1mg

价格：934,1674,3090,5057

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgM

克隆号：HM40-3

荧光染料：FITC

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	1 μg/test(Flow)

产品详细信息Description: The HM40-3 monoclonal antibody reacts with mouse and rat CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.Applications Reported: The HM40-3 antibody has been reported for use in flow cytometric analysis.Applications Tested: The HM40-3 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.

数据

CD40 Antibody (11-0402-81) in FlowStaining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) PerCP-eFluor® 710 (Product # 46-0452-82) and 0.5 µg of Anti-Mouse/Rat CD40 FITC. Total viable cells were used for analysis.

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参考文献：

1.American journal of translational researchThiol peroxiredoxin, a novel allergen fromBombyx mori, modulates functions of macrophages and dendritic cells."11-0402 was used in Flow cytometry/Cell sorting to study how thiol peroxiredoxin, a novel allergen from Bombyx mori, modulates functions of macrophages and dendritic cells."2.Nature communicationsEnvironmental sensing by mature B cells is controlled by the transcription factors PU.1 and SpiB."Published figure using CD40 monoclonal antibody (Product # 11-0402-82) in Flow Cytometry"3.European journal of immunologyToll like Receptor 2 engagement on CD4+T cells promotes TH9 differentiation and function."11-0402 was used in Flow cytometry/Cell sorting to study the role of T-cell expressed TLR2 on CD4+ T-cell differentiation and function."AuthorsKarim AF,Reba SM,Li Q,Boom WH,Rojas RE4.Scientific reportsDer f 31, a novel allergen from Dermatophagoides farinae, activates epithelial cells and enhances lung-resident group 2 innate lymphoid cells."Published figure using CD40 monoclonal antibody (Product # 11-0402-82) in Flow Cytometry"5.Brain and behaviorTargeting resolution of neuroinflammation after ischemic stroke with a lipoxin A4analog: Protective mechanisms and long-term effects on neurological recovery."11-0402 was used in Flow cytometry/Cell sorting to identify the endogenous lipoxin A4 pathway as a promising therapeutic target for the treatment of ischaemic stroke."AuthorsHawkins KE,DeMars KM,Alexander JC,de Leon LG,Pacheco SC,Graves C,Yang C,McCrea AO,Frankowski JC,Garrett TJ,Febo M,Candelario-Jalil E

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