SCF Human ELISA Kit/人SCF ELISA试剂盒
货号：EHKITLG,EHKITLGX5,EHKITLGX10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 2 pg/mL |
| 检测范围 | 2.74-2000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | SCF |
| Protein aliases | c-Kit ligand, familial progressive hyperpigmentation 2, Kit ligand, Mast cell growth factor, MGF, Stem cell factor, SCF, sKITLG, Soluble KIT ligand, steel factor |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | DCUA, DFNA69, FPH2, FPHH, Kitl, KITLG, KL-1, MGF, SCF, SF, SHEP7 |
| Gene ID | (Human) 4254 |
| Gene symbol | KITLG |
| UniProt ID | (Human) P21583 |
The Human Stem Cell Factor/KITLG (Hu SCF) ELISA quantitates Hu SCF in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu SCF.人干细胞因子/ KITLG（人SCF）ELISA定量了人血清，血浆或细胞培养基中的人SCF。 该检测试剂盒将专门识别天然和重组人SCF。Principle of the method 方法原理The Human SCF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人SCF固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.干细胞因子（SCF）是c-Kit癌基因的配体，由气道中的各种结构细胞和炎症细胞表达。 c-Kit受体与SCF的结合导致受体的二聚化，并激活诸如PI-3，PLC-γ，Jak / STAT和MAP激酶等信号通路。 SCF的表达导致肥大细胞存活的诱导以及组胺，促炎细胞因子和趋化因子的表达和释放。 SCF / c-Kit途径的抑制导致组胺水平降低，肥大细胞和嗜酸性粒细胞浸润，IL-4产生以及气道高反应性降低，表明该途径可能是炎性疾病（例如哮喘）的有用治疗靶标。 已知存在至少两个SCF同工型。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
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