CD45.1 Monoclonal Antibody (A20), FITC

货号：11-0453-81,11-0453-82,11-0453-85

规格：50μg,100μg,500μg

价格：822,1416,3035

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：A20

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), FITC
浓度：	0.5 mg/mL	用法：	0.5 μg/test(Flow)

产品详细信息Description: The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains. The strains that express CD45.1 include SJL/J, DA, STS/A and RIII. CD45.1 is expressed by all leukocytes in these strains.Applications Reported: The A20 antibody has been reported for use in flow cytometric analysis.Applications Tested: The A20 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they shareidentical transmembrane and cytoplasmic domains. These CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.

数据

CD45.1 Antibody (11-0453-81) in FlowStaining of BALB/c (left) or SJL (right) splenocytes with 0.25 µg of Mouse IgG2a kappa Isotype Control FITC (Product # 11-4724-42) or 0.25 µg of Anti-Mouse CD45.1 FITC (filled histogram). Total viable cells were used for analysis.

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参考文献：

1.Scientific reportsAn immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages."Published figure using CD45.1 monoclonal antibody (Product # 11-0453-82) in Flow Cytometry"2.Molecular and cellular biologyNeutrophil and Macrophage Cell Surface Colony-Stimulating Factor 1 Shed by ADAM17 Drives Mouse Macrophage Proliferation in Acute and Chronic Inflammation."11-0453 was used in Flow cytometry/Cell sorting to identify a significant defect in macrophage proliferation in mice lacking leukocyte transmembrane protease, ADAM17."AuthorsTang J,Frey JM,Wilson CL,Moncada-Pazos A,Levet C,Freeman M,Rosenfeld ME,Stanley ER,Raines EW,Bornfeldt KE3.Stem cell reportsTGF-β1 Negatively Regulates the Number and Function of Hematopoietic Stem Cells."11-0453 was used in Flow cytometry/Cell sorting to examine the effect of transforming growth factor Β1 on haematopoietic stem cell function."AuthorsWang X,Dong F,Zhang S,Yang W,Yu W,Wang Z,Zhang S,Wang J,Ma S,Wu P,Gao Y,Dong J,Tang F,Cheng T,Ema H4.Nature medicineAdrenergic nerve degeneration in bone marrow drives aging of the hematopoietic stem cell niche."11-0453 was used in Flow cytometry/Cell sorting to determine whether haematopoietic stem cell ageing depends on bone marrow innervation by the sympathetic nervous system."AuthorsMaryanovich M,Zahalka AH,Pierce H,Pinho S,Nakahara F,Asada N,Wei Q,Wang X,Ciero P,Xu J,Leftin A,Frenette PS5.CellInitiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells."Published figure using CD45.1 monoclonal antibody (Product # 11-0453-82) in Flow Cytometry"

技术参数

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