CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), FITC

货号：11-0541-81,11-0541-82

规格：50μg,100μg

价格：851,1599

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：YN1/1.7.4

荧光染料：FITC

类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), FITC
浓度：	0.5 mg/mL	用法：	1 μg/test(Flow)

产品详细信息Description: The YN1/1.7.4 monoclonal antibody reacts with mouse CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes, dendritic cells, and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligands CD11a and CD11b is important in the inflammatory response. The YN1/1.7.4 monoclonal antibody recognizes a different epitope than the eBioKAT-1 monclonal antibody.Applications Reported: The YN1/1.7.4 antibody has been reported for use in flow cytometric analysis.Applications Tested: The YN1/1.7.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

数据

CD54 (ICAM-1) Antibody (11-0541-81) in FlowStaining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.5 µg of Anti-Mouse CD54 (ICAM-1) FITC (filled histogram). Total viable cells were used for analysis.

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参考文献：

1.International journal of molecular sciencesLow-Dose Radiotherapy Has No Harmful Effects on Key Cells of Healthy Non-Inflamed Joints."Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 11-0541-82) in Flow Cytometry"2.Cell stem cellConstitutively Active SMAD2/3 Are Broad-Scope Potentiators of Transcription-Factor-Mediated Cellular Reprogramming."Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 11-0541-82) in Flow Cytometry"3.Infection and immunityCyclic AMP-Elevating Capacity of Adenylate Cyclase Toxin-Hemolysin Is Sufficient for Lung Infection but Not for Full Virulence of Bordetella pertussis."11-0541 was used in Flow cytometry/Cell sorting to construct the first B. pertussis mutant secreting a CyaA toxin having an intact capacity to deliver the AC enzyme into CD11b-expressing host phagocytes but impaired in formation of cell-permeabilizing pores and defective in cAMP elevation in CD11b- cells."AuthorsSkopova K,Tomalova B,Kanchev I,Rossmann P,Svedova M,Adkins I,Bibova I,Tomala J,Masin J,Guiso N,Osicka R,Sedlacek R,Kovar M,Sebo P4.Stem cell reportsReprogramming Roadblocks Are System Dependent."Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 11-0541-82) in Flow Cytometry"5.CirculationMicroRNA-155 Exerts Cell-Specific Antiangiogenic but Proarteriogenic Effects During Adaptive Neovascularization."11-0541 was used in Flow cytometry/Cell sorting to identify regulatory microRNAs involved in the modulation of neovascularisation after femoral artery occlusion in mice."AuthorsPankratz F,Bemtgen X,Zeiser R,Leonhardt F,Kreuzaler S,Hilgendorf I,Smolka C,Helbing T,Hoefer I,Esser JS,Kustermann M,Moser M,Bode C,Grundmann S

技术参数

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