IL-2 Human ELISA Kit, High Sensitivity/人IL-2高灵敏ELISA试剂盒
货号：BMS221HS
规格：96 tests
价格：7428
产品类型：High-Sensitivity ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 0.4 pg/mL |
| 检测范围 | 0.94-60.0 pg/mL |
样本类型/体积 | 血浆 50 μL 血清 50 μL 上清液 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr |
| 批间变异系数 | 6.2% |
| 批内变异系数 | 5.7% |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 样品稀释液 - 分析缓冲浓缩液 - 生物素化检测抗体 - 放大稀释浓缩液 - 放大试剂Ⅰ - 放大试剂Ⅱ - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | IL-2 |
| Protein aliases | Aldesleukin, B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, Binetrakin, BSF-1, IL-2, T-cell growth factor, TCGF, IL-4, interleukin 4 variant 2, Interleukin-2, Interleukin-4, involved in regulation of T-cell clonal expansion, Lymphocyte stimulatory factor 1, Pitrakinra, T cell growth factor |
物种（Tested） | 人 |
试剂盒类型 | High Sensitivity Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene ID | (Human) 3558,(Human) 3565 |
| Gene aliases | BCGF-1, BCGF1, BSF-1, BSF1, IL-2, IL-4, IL2, IL4, lymphokine, TCGF |
| Gene symbol | IL2, IL4 |
UniProt ID | (Human) P60568,(Human) P05112 |
| The Human High Sensitivity Interleukin 2 (HS IL-2) ELISA quantitates Hu HS IL-2 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HS IL-2.人白介素2（IL-2）高灵敏ELISA定量了人类血清，血浆，缓冲液或细胞培养基中的人IL-2。 该检测试剂盒将专门识别天然和重组的人IL-2。Principle of the method 方法原理The Human HS IL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A special amplification reagent step is added to achieve the high sensitivity capability. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-2高灵敏度固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 添加了特殊的扩增试剂步骤以实现高灵敏度。 经过孵育和洗涤步骤以去除未结合物质的微孔板后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.白介素2（IL-2）是一种免疫调节性细胞因子，对于活化T细胞的增殖，B细胞，自然杀伤细胞，单核细胞和巨噬细胞的分化非常重要。 IL-2通过IL-2受体（IL-2R）发出信号，IL-2R是一种异源三聚体蛋白复合物，其γ链也被白介素4（IL-4）和白介素7（IL-7）共享。 IL-2基因在成熟胸腺细胞中的表达是单等位基因，代表了控制单个基因精确表达的异常调节模式。 在小鼠中类似于IL-2的基因的靶向破坏会导致溃疡性结肠炎样疾病，这提示该基因在对抗原刺激的免疫反应中起着至关重要的作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
| 数据： |
| IL-2 Human ELISA Kit, High SensitivityRepresentative standard curve for Human IL-2 ELISA |
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