Mouse IgG2a kappa Isotype Control (eBM2a), PerCP-eFluor 710

货号： 46-4724-80,46-4724-82

规格：25 μg,100 μg

价格：1029,2365

产品类型：小鼠同型对照

品牌：Thermo Fisher

物种：其它

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：eBM2a

荧光染料：PerCP-eFluor™ 710

类型:		同型对照：
浓度：	0.2 mg/mL	用法：	Assay-Dependent (Ctrl)；Assay-Dependent (Flow)

产品详细信息Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.Applications Reported: PerCP-eFluor® 710 Mouse IgG2a, K Isotype Control has been reported for use in flow cytometric analysis.Applications Tested: PerCP-eFluor® 710 Mouse IgG2a Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells.Use at the same concentration as experimental antibody.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

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参考文献：

1.PloS oneEffects of prenatal low protein and postnatal high fat diets on visceral adipose tissue macrophage phenotypes and IL-6 expression in Sprague Dawley rat offspring."46-4724 was used in Flow cytometry/Cell sorting to show that both LP and HE diets impact adipose tissue macrophage phenotype by affecting the ratio of CD11c+CD206+ ATMs and the expression of IL-6."AuthorsXie L,Zhang K,Rasmussen D,Wang J,Wu D,Roemmich JN,Bundy A,Johnson WT,Claycombe K2.PloS oneInnate Response Activator (IRA) B Cells Reside in Human Tonsils and Internalize Bacteria In Vitro."46-4724 was used in Flow cytometry/Cell sorting to study the role for hIRA B cells in the effector immune response to infections in tonsils."AuthorsChiappini N,Cantisani R,Pancotto L,Ruggiero P,Rosa D,Manetti A,Romano A,Montagnani F,Bertholet S,Castellino F,Del Giudice G

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