Phosphotyrosine Monoclonal Antibody (pY20), AP

货号：03-7722

规格：100μg

价格：4809

产品类型：流式抗体

品牌：Thermo Fisher

物种：其它

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：pY20

荧光染料：AP(碱性磷酸酶)

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	Assay Dependent (ELISA)；1:1,000(WB)

产品详细信息This antibody is known to show reactivity towards phosphothreonine.靶标信息The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

数据

Phosphotyrosine Antibody (03-7722)Angiogenesis 2010 -Fig. 3 NVP-BHG712 inhibits endogenous EphB4 autophosphorylation in lung tissue of mice and has micromolar exposure in plasma and tissues. NVP-BHG712 has been dosed orally in mice (50 mg/kg). Mice have been sacrificed at time points after oral compound administration as indicated. a To monitor NVP-BHG712 concentration and EphB4 autophosphorylation in lung tissue, one lung lobe were lysed for western analysis and the other lung lobe was used for pharmacokinetic analysis. EphB4 protein was immuno-precipitated from lung tissue lysates and analyzed by western using anti-phospho-tyrosine antibodies. Membranes were stripped and reprobed with a specific anti-EphB4 antibody to ensure equal protein loading. Corresponding NVP-BHG712 concentrations are indicated below each lane. b NVP-BHG712 concentrations in plasma ( filled circle ), lung tissue ( filled square ) and liver tissue ( open circle ) were analyzed by HPLC-UV as described in the material and method chapter

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参考文献：

1.Cancer discoveryPolyclonal SecondaryFGFR2Mutations Drive Acquired Resistance to FGFR Inhibition in Patients with FGFR2 Fusion-Positive Cholangiocarcinoma."37722 was used in ELISA to determine the molecular basis for acquired resistance to the fibroblast growth factor receptor inhibitor BGJ398"AuthorsGoyal L,Saha SK,Liu LY,Siravegna G,Leshchiner I,Ahronian LG,Lennerz JK,Vu P,Deshpande V,Kambadakone A,Mussolin B,Reyes S,Henderson L,Sun JE,Van Seventer EE,Gurski JM,Baltschukat S,Schacher-Engstler B,Barys L,Stamm C,Furet P,Ryan DP,Stone JR,Iafrate AJ,Getz G,Porta DG,Tiedt R,Bardelli A,Juric D,Corcoran RB,Bardeesy N,Zhu AX2.European journal of haematologyPhosphorylation of Tyr245 in the open-inhibited state of Abelson kinase does not induce downstream signaling."03-7722 was used in ELISA to investigate the role of Tyr245 phosphorylation in Abelson kinase function"AuthorsSkora L,Kempf D,Mestan J,D'Orazio D,Jahnke W3.Proceedings of the National Academy of Sciences of the United States of AmericaNMR reveals the allosteric opening and closing of Abelson tyrosine kinase by ATP-site and myristoyl pocket inhibitors."03-7722 was used in an ELISA assay to analyse the solution conformations of Abelson tyrosine kinase (c-Abl) and c-Abl complexes with ATP-site and allosteric inhibitors."AuthorsSkora L,Mestan J,Fabbro D,Jahnke W,Grzesiek S4.AngiogenesisThe small molecule specific EphB4 kinase inhibitor NVP-BHG712 inhibits VEGF driven angiogenesis."03-7722 was used in ELISA to develop and characterize an inhibitor of EphB4 kinase"5.CancersIncreased Level of Phosphorylated ShcA Measured by Chemiluminescence-Linked Immunoassay Is a Predictor of Good Prognosis in Primary Breast Cancer Expressing Low Levels of Estrogen Receptor."03-7722 was used in an ELISA assay to study the link between SH2 domain-containing adaptor protein activation, the estrogen receptor, and primary breast cancer prognosis."AuthorsCicenas J,Küng W,Eppenberger U,Eppenberger-Castori S

技术参数

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