IFN beta Mouse ELISA Kit/小鼠IFN β ELISA试剂盒
货号：424001
规格：96 tests
价格：10030
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
| 检测范围 | 15.6-1000 pg/ml |
样本类型/体积 | 上清液 100 μL |
操作时间 | 1 hr 20 min |
测试时间 | 3 hr 15 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <8% |
| 批内变异系数（CV） | <8% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 tests |
组份 | - 预包被96孔板 - 检测抗体浓缩液 - +抗IgG-HRP浓缩液 - 浓缩稀释剂 - 标准品 - 洗涤缓冲浓缩液 - 底物溶液 - 样品稀释液 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | IFN beta |
| Protein aliases | IFN-beta, Interferon beta |
物种（Tested） | 小鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | HRP |
| Gene aliases | Ifb, IFN-beta, IFNB, Ifnb1 |
| Gene ID | (Mouse) 15977 |
| Gene symbol | Ifnb1 |
| UniProt ID | (Mouse) P01575 |
| The Mouse Interferon beta (Ms IFNβ) ELISA quantitates Ms IFNβ in mouse cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IFNβ.小鼠干扰素β（小鼠IFNβ）ELISA定量检测小鼠细胞培养基中的小鼠IFNβ。 该检测试剂盒将专门识别天然和重组小鼠IFNβ。Principle of the method 方法原理The Mouse IFNβ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠IFNβ固相夹心ELISA（酶联免疫吸附测定）旨在测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by Natural IFN-producing cells (IPCs) as part of the host immune response. IFNs can also inhibit the development of tumors. IFN-beta binding results in the activation of the tyrosine kinases Jak1 and Tyk2, phosphorylation of members of the STAT family of transcription factors, and the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-beta. IFN-beta is currently used clinically for treatment of tumors, infections and multiple sclerosis.I型干扰素（IFN-α/β）主要是由于天然干扰素产生细胞（IPC）对病毒感染的反应而产生，是宿主免疫反应的一部分。 干扰素也可以抑制肿瘤的发展。 IFN-β结合导致酪氨酸激酶Jak1和Tyk2活化，转录因子STAT家族成员的磷酸化以及免疫应答基因的转录和表达。 最近，人们发现了Toll样受体（TLR）家族的几个成员可以刺激IFN-β的产生。 IFN-β目前在临床上用于治疗肿瘤，感染和多发性硬化症。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
| 数据： |
IFN beta Mouse ELISA KitRepresentative standard curve for the Mouse IFN-β ELISA |
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