NFkB p65 Monoclonal Antibody (2A12A7)/NFkB p65单克隆抗体（2A12A7）
货号：33-9900
规格：100 µg
价格：4809
产品类型：抗体和染料
品牌：Thermo Fisher
抗原：Recombinant protein (~175 amino acids) from the C-
物种：人/小鼠
宿主：小鼠
抗体亚型：其它
克隆号：2A12A7
荧光染料：其它
| 类型: | 单抗 | 同型对照： | |
| 浓度： | 0.5 mg/mL | 用法： | 3 µg/mL(ChIP)；Assay Dependent(ELISA)；Assay Dependent(GS)；Assay Dependent(IHC (P))；1 µg/mL(WB) |
靶标信息Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. Phosphorylation of p65 at Ser 536 located in the carboxy-terminal transactivation domains (TAD) leads to nuclear localization of the transcriptionally active complex and NF-kB mediated transactivation of several downstream genes. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.
| 数据： |
NFkB p65 Antibody (33-9900) in IHC (P)Immunohistochemistry analysis of NF-kappa-B (p65) showing staining in the Cytoplasm and Nucleus of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a NF-kappa-B (p65) mouse monoclonal antibody (Product # 33-9900) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. NFkB p65 Antibody (33-9900)British journal of pharmacology 2018 -Published figure using NFkB p65 monoclonal antibody (Product # 33-9900) |
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