H3K9ac Monoclonal Antibody/H3K9ac单克隆抗体
货号：MA5-23517
规格：50 µg
价格：4532
产品类型：细胞分选
品牌：Alfa Aesar
| 类型: | 单抗 | 同型对照： | |
| 浓度： | 1 mg/mL | 用法： | 1:500(ICC)；1:500(IF)；1:3000(pep-ELISA)；1:1,000(WB)；1:2,000(Array) |
靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
| 数据： |
H3K9ac Antibody (MA5-23517) in IFImmunofluorescent detection of Acetyl-Histone H3 (Lys9) in HeLa cells stained with Acetyl-Histone H3 (Lys9) monoclonal antibody (Product # MA5-23517). Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/Triton X-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9ac antibody (left) at a dilution of 1:500 in blocking solution followed by an anti-mouse antibody conjugated to AlexaFluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. H3K9ac Antibody (MA5-23517) in ICCImmunofluorescence analysis of Acetyl-Histone H3 (Lys9) was performed using 70% confluent log phase HeLa cells treated with sodium butyrate. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Acetyl-Histone H3 (Lys9) Mouse Monoclonal Antibody (Product # MA5-23517) at 1:250 dilution in 0.1% BSA, incubated overnight at 4 degree Celsius and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents the untreated cells showing relatively lower expression of Acetyl-Histone H3 (Lys9). Panel f shows control cells with no primary antibody to assess background. The images were captured at 60X magnification. |
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