H3K9me2 Polyclonal Antibody
货号：49-1007
规格：50 µg
价格：4809
产品类型：标签抗体
品牌：Thermo Fisher
抗原：Raised against the region of the histone H3 contai
物种：人
宿主：兔
抗体亚型：IgG
荧光染料：其它
| 类型: | 一抗 | 同型对照： | |
| 浓度： | 1.15 mg/mL | 用法： | 1:20,000(DB)；1:1000(ELISA)；1:500(ICC)；1:500(IF)；1:1,000(WB)；0.25μg/mL(Array) |
靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
| 数据： |
H3K9me2 Antibody (49-1007) in IFImmunofluorescence analysis of Di-Methyl-Histone H3 (Lys9)was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Rabbit Polyclonal Antibody (Product # 49-1007) at 1:250 dilution in 0.1% BSA and incubated overnight at 4°C and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. H3K9me2 Antibody (49-1007) in IFMouse NIH3T3 cells were stained with the anti-H3K9me2 antibody (Product # 49-1007) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K9me2 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. |
| 推荐产品： |
| 二抗荧光二抗免疫组化一抗标签抗体 |
QQ:1749072012