H3K36me2 Monoclonal Antibody (T.571.7), ChIP-Verified/H3K36me2单克隆抗体（T.571.7），ChIP认证
货号：MA5-14867
规格：100 µL
价格：1
产品类型：抗体和染料
品牌：Thermo Fisher
抗原：Synthetic peptide corresponding to the amino termi
物种：其它
宿主：兔
抗体亚型：IgG
克隆号：T.571.7
荧光染料：其它
| 类型: | 单抗 | 同型对照： | |
| 浓度： | | 用法： | 1-5 µg(ChIP)；1:100(ICC)；1:100(IF)；1:25(IHC (P))；1 µg/mL(WB)；0.25 µg/mL(Array) |
产品详细信息It is not recommended to aliquot this antibody.This antibody is not cross-reactive with di-methylated histone H3 Lys4, Lys9, Lys27, Lys79 or methylated histone H4 Lys20.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
| 数据： |
H3K36me2 Antibody (MA5-14867) in IFImmunofluorescent analysis of Di-Methyl-Histone H3 (Lys36) in HeLa cells using a Di-Methyl-Histone H3 (Lys36) monoclonal antibody (Product # MA5-14867) (green). Actin filaments are labeled with a fluorescent red phalloidin. H3K36me2 Antibody (MA5-14867) in ChIPK-MetStat Panel, SNAP-ChIP™ Spike-in (Product # A47356), a proprietary technology developed byEpiCypher™was used to analyze the performance of H3K36me2 antibody (Product # MA5-14867) in ChIP. SNAP-ChIP panels consist of a pool of DNA-barcoded recombinant nucleosomes harboring histone PTMs that are spiked-in to a ChIP reaction to assess efficiency and specificity of the antibody. The K-MetStat panel includes an unmodified control plus nucleosomes containing histones with mono, di and tri-methyl forms of lysine residues on histone H3 and H4 as shown on the x-axis. Recovery of each unique DNA-barcoded nucleosome is quantified to determine how much of each PTM is immunoprecipitated in the ChIP reaction (for more information seereference). H3K36me2 antibody was tested in native ChIP with 3 µg K-562 cell chromatin and 3 µg antibody. Specificity (left Y-axis) was determined by qPCR to each modified nucleosome in the panel (X-axis). Black bar represents antibody efficiency (right Y-axis; log scale) and indicates percentage of the barcoded nucleosome target immunoprecipitated relative to Input. All bars represent mean ± SEM. |
| 推荐产品： |
| 二抗荧光二抗免疫组化一抗标签抗体 |
QQ:1749072012