H3K9me3 Polyclonal Antibody/H3K9me3多克隆抗体

货号：PA5-31910

规格：100 µL

价格：5942

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to a region within

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.2 mg/mL	用法：	Assay Dependent(ChIP)；1:100-1:1,000(ICC)；1:100-1:1,000(IF)；1:100-1:1,000(IHC (P))；Assay Dependent(IM)；1:100-1:1000(IP)；1:500-1:3,000(WB)；0.25 µg/mL(Array)

产品详细信息Recommended positive controls: 293T, A431, HeLa, HepG2.Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K9me3 Antibody (PA5-31910) in IFImmunofluorescence analysis of Tri-Methyl-Histone H3 (Lys9) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Tri-Methyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody (Product # PA5-31910) at 5µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. H3K9me3 Antibody (PA5-31910) in IFImmunofluorescent analysis of Tri-Methyl-Histone H3 (Lys9) in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min, using a Tri-Methyl-Histone H3 (Lys9) polyclonal antibody (Product # PA5-31910). Green: Primary antibody at a dilution of 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining.

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