The Vericheck ddPCR Empty-Full Capsid Kit enables a quick and precise measurement of the empty-full capsid ratio that is needed to produce AAV vectors. The empty-full capsid ratio is a Critical Quality Attribute (CQA) in AAV vector production since high amounts of empty capsids may cause unwanted immunotoxicity. This kit simultaneously provides consistent and reliable capsid content measurements across the AAV production process with high precision, multi-sample type compatibility, low-sample input, fast turnaround time, and easy data analysis.

Features & Benefits

Sample type compatibility — crude lysate and purified

Low sample input — only 106 virus particles required to get results

Fast turnaround time — up to 14 samples per plate in less than 1 day

High precision — coefficient of variation (CV) less than 5%

Simplified data analysis – streamlined process providing simultaneous results for genome titer, capsid titer, and empty-full ratio

Applications & Uses

Simply and precisely measure empty-full ratio with one assay

Optimized to provide precision throughout the AAV characterization process

Instrument Compatibility

(Bundles or different component sets) Catalog Number

QX ONE Droplet Digital PCR System 12006536

QX200 AutoDG Droplet Digital PCR System consisting of:

QX200 Droplet Reader

Automated Droplet Generator 1864100

1864003

1864101

QX600 Droplet Digital PCR Reader 12013328

QX Manager Software, Regulatory Edition 12012172

QX Manager Software, Standard Edition 12012171

QX ONE Software, Regulatory Edition 12012078

QX Manager, Premium Edition 12018108