Phospho-IKK alpha/beta (Ser180, Ser181) Polyclonal Antibody

货号：PA5-104696

规格：100 µL

价格：4643

产品类型：标签抗体

品牌：Thermo Fisher

抗原：A synthesized peptide derived from human IKK- alph

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	1:100-1:500(ICC);1:100-1:500(IF);1:50-1:200(IHC(P))；1:500-1:2000(WB)

靶标信息IKK alpha/beta is encoded by CHUK (conserved helix-loop-helix ubiquitous kinase) and IKKB (inhibitor of kappa light polypeptide gene enhancer in B-cells) gene located in chromosome 10 and 8 respectively in humans. Upon stimulation by agents that activate the NF-kB pathway such as TNF alpha, the two subunits of IKK complex, IKK alpha and IKK -beta alongside a regulator NEMO (IKK gamma) phosphorylates IkB proteins. This targets the later for ubiquitin mediated degradation and phosphorylation of NF-kB leading to subsequent translocation to the nucleus. Phosphorylation at serine 176 and 180 located in the activation loop causes a confirmation change that is required for the activation of the kinases.

数据：

Phospho-IKK alpha/beta (Ser180, Ser181) Antibody (PA5-104696) in IFImmunofluorescent analysis of Phospho-IKK alpha/beta (Ser180, Ser181) in HepG2. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with Phospho-IKK alpha/beta (Ser180, Ser181) polyclonal antibody (Product # PA5-104696) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600. Phospho-IKK alpha/beta (Ser180, Ser181) Antibody (PA5-104696) in IHC (P) Immunohistochemistry analysis of paraffin-embedded Phospho-IKK alpha/beta (Ser180, Ser181) in mouse lung tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-IKK alpha/beta (Ser180, Ser181) polyclonal antibody (Product # PA5-104696) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit. Phospho-IKK alpha/beta (Ser180, Ser181) Antibody (PA5-104696) in WB Western blot analysis of Phospho-IKK alpha/beta (Ser180, Ser181) in TNF treated HepG2 cell lysate (right lane: treated with the antigen-specific peptide). Samples were incubated with Phospho-IKK alpha/beta (Ser180, Ser181) polyclonal antibody (Product # PA5-104696).

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