Phospho-SETD8 (Ser100) Polyclonal Antibody

货号：PA5-105328

规格：100 µL

价格：4711

产品类型：标签抗体

品牌：Thermo Fisher

抗原：A synthesized peptide derived from human KMT5A aro

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	1:200(IF);1:50-1:200(IHC(P));1:500-1:2000(WB)

靶标信息SETD8 is a histone methyltransferase that specifically monomethylates 'Lys-20' of histone H4. H4 'Lys-20' monomethylation is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. The protein mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It is required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. It is also involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones.

数据：

Phospho-SETD8 (Ser100) Antibody (PA5-105328) in IFImmunofluorescent analysis of Phospho-SETD8 (Ser100) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-SETD8 (Ser100) polyclonal antibody (Product # PA5-105328) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green). Phospho-SETD8 (Ser100) Antibody (PA5-105328) in IHC (P)Immunohistochemistry analysis of paraffin-embedded Phospho-SETD8 (Ser100) in rat liver tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-SETD8 (Ser100) polyclonal antibody (Product # PA5-105328) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit. Phospho-SETD8 (Ser100) Antibody (PA5-105328) in WBWestern blot analysis of Phospho-SETD8 (Ser100) in 293T cell lysate (Lane 1: treated with phospho-blocking peptide; Lane 2: treated with non-phospho-blocking peptide). Samples were incubated with Phospho-SETD8 (Ser100) polyclonal antibody (Product # PA5-105328).

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