14-3-3 sigma Polyclonal Antibody
货号:PA5-87755
规格:100 µL
价格:4732
产品类型:标签抗体
品牌:Thermo Fisher
抗原:Recombinant fusion protein containing a sequence c
物种:人/小鼠/大鼠
宿主:兔
抗体亚型:IgG
荧光染料:其它
类型: | 一抗 | 同型对照: | |
浓度: | 1 .69 mg/mL | 用法: | 1:50-1:200(IHC(P));1:50-1:200(ICC);1:50-1:200(IF);1:500-1:2000(WB) |
产品详细信息Immunogen sequence: MERASLIQKA KLAEQAERYE DMAAFMKGAV EKGEELSCEE RNLLSVAYKN VVGGQRAAWR VLSSIEQKSN EEGSEEKGPE VREYREKVET ELQGVCDTVL GLLDSHLIKE AGDAESRVFY LKMKGDYYRY LAEVATGDDK KRIIDSARSA YQEAMDISKK EMPPTNPIRL GLALNFSVFH YEIANSPEEA ISLAKTTFDE AMADLHTLSE DSYKDSTLIM QLLRDNLTLW TADNAGEEGG EAPQEPQS; Positive Samples: HT-29, MCF7; Cellular Location: Cytoplasm, Nucleus靶标信息This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis.
数据: |
14-3-3 sigma Antibody (PA5-87755) in ICCImmunocytochemistry-Immunofluorescence analysis of 14-3-3 sigma was performed in MCF-7 cells using 14-3-3 sigma Polyclonal Antibody (Product # PA5-87755).14-3-3 sigma Antibody (PA5-87755) in IHCImmunohistochemistry analysis of 14-3-3 sigma in paraffin-embedded rat kidney using 14-3-3 sigma Polyclonal Antibody (Product # PA5-87755) at a dilution of 1:100.14-3-3 sigma Antibody (PA5-87755) in WBWestern Blot analysis of 14-3-3 sigma in extracts of various cell lines using 14-3-3 sigma Polyclonal Antibody (Product # PA5-87755) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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