DH5α Competent Cells/DH5α 感受态细胞
货号：EC0112
规格：10 x 100 µL
价格：1205
产品类型：感受态细胞
品牌：Thermo Fisher
| Thermo Scientific DH5α Competent Cells are high efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal. RecA1 and endA1 mutations in DH5α cells improve insert stability and quality of the extracted plasmid DNA.• High transformation efficiency: >1x109cfu/µg pUC19 DNA• Suitable for routine and high-throughput cloning applications• Genetic markers that allow for blue/white colony screening• Convenient two reactions per vial packaging• S.O.C. Outgrowth medium includedApplicationsDH5α Competent Cells are suitable for variety of applications requiring high transformation efficiency:• Efficient DNA cloning derived from PCR, cDNA-generation reactions• recA1 marker facilitates working with difficult to transform DNA• Ideal for generation of cDNA libraries using plasmid-derived vectors• Site-directed mutagenesisGenotypeF– φ80lacZΔ M15 Δ (lacZYA-argF)U169 recA1 endA1 hsdR17(rK– mK+)phoA supE44λ-thi–1 gyrA96 relA1 |
优点： |
| - High transformation efficiency: >1x109cfu/µg pUC19 DNA- Suitable for routine and high-throughput cloning applications- Genetic markers that allow for blue/white colony screening- Convenient two reactions per vial packaging- S.O.C. Outgrowth medium included |
数据： |
| 图1.Transformation efficiency of DH5α Competent CellsThermo Scientific DH5α competent cells provide high transformation efficiency. The transformation efficiencies of DH5α competent cells and NEB 5-alpha competent E. coli were compared using pUC19 DNA and following the manufacturers’ recommended protocols. The transformation efficiency specification for DH5α competent cells is 1.0 x 109CFU/µg DNA (represented by the red dashed line). The values shown are the averages of five transformations. |
组成成分： |
| ▪DH5α Competent Cells ▪pUC19 DNA (10 pg/µL) ▪S.O.C. Medium |
相关产品： |
▪DH5αCompetent Cells for Subcloning/DH5α 感受态细胞用于亚克隆（货号#EC0111) ▪DH5α Competent Cells/DH5α感受态细胞（货号#EC0112）▪DH10B Competent Cells/DH10B感受态细胞（货号#EC0113）▪BL21(DE3) Competent Cells/BL21(DE3)感受态细胞（货号#EC0114） |
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