Lambda DNA/HindIII Marker
货号:SM0101,SM0102
规格:5 x 50 µg,25 x 50 µg
价格:513,2041
产品类型:DNA电泳
品牌:Thermo Fisher
Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.The marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, and it can be labeled radioactively or non-radioactively withKlenow FragmentorKlenow Fragment, exo-with the fill-in reaction (seeResources for Protocols).NoteThe cohesive ends (the 12 nt cos site of lambda DNA) of fragments indicated may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5 minutes and then cooling on ice for 3 minutes. |
优点: |
- Sizing and approximate quantification of large DNA fragments-Sharp bands-Supplied with loading dye for sample DNA |
数据: |
图1.Lambda DNA/HindIII Marker |
组成成分: |
▪Lambda DNA/HindIII Marker, 2,▪6X DNA Loading Dye |
相关产品: |
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技术参数 产品优点 1.Sizing and approximate quantification of large DNA fragments
2.Sharp bands
3.Supplied with loading dye for sample DNA