Lambda DNA/EcoRI plus HindIII Marker
货号：SM0191,SM0192
规格：5 x 50 µg,25 x 50 µg
价格：487,1956
产品类型：DNA电泳
品牌：Thermo Fisher
| Thermo Scientific Lambda DNA/EcoRI+HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.Lambda DNA/EcoRI+HindIII Marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, and it can be labeled radioactively or non-radioactively withKlenow fragmentorKlenow Fragment, exo-with the fill-in reaction (seeResources for Protocols).NoteThe cohesive ends (the 12nt cos site of lambda DNA) of fragments indicated may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5minutes and then cooling on ice for 3minutes.The shortest fragments (oblique) are not visible in standard electrophoresis. Fragment lengths are predicted by computer analysis of respective DNA sequences. |
优点： |
| Highlights- Sizing and approximate quantification of large DNA fragments- Sharp bands- Supplied with loading dye for sample DNA |
组成成分： |
| ▪Lambda DNA/EcoRI+HindIII Marker, 3▪6X DNA Loading Dye |
相关产品： |
| ▪Lambda DNA/HindIII Marker（货号#SM0101/SM0102）▪Lambda DNA/HindIII Marker, ready-to-use（货号#SM0103）▪Lambda DNA/EcoRI plus HindIII Marker, ready-to-use（货号#SM0193）▪pUC19 DNA/MspI (HpaII) Marker（货号#SM0221）▪pUC19 DNA/MspI (HpaII) Marker, ready-to-use （货号#SM0223）▪GeneRuler 100 bp DNA Ladder （货号#SM0241）▪GeneRuler 100 bp DNA Ladder, ready-to-use（货号#SM0243）▪DNA电泳 |
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