| Thermo Scientific Lambda DNA is digested to completion with the appropriate Thermo Scientitific restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.The Thermo Scientific marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, it can be labeled radioactively or non-radioactively withKlenow fragmentorKlenow Fragment, exo-with the fill-in reaction (seeRecources for Protocols).Note• The cohesive ends (the 12nt cos site of lambda DNA) of fragments indicated may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5 minutes and then cooling on ice for 3minutes. |
优点： |
| - Sizing and approximate quantification of large DNA fragments- Sharp bands- Supplied with loading dye for sample DNA |
组成成分： |
| ▪Lambda DNA/EcoRI Marker, 1▪6X DNA Loading Dye |
相关产品： |
| ▪Lambda DNA/HindIII Marker（货号#SM0101/SM0102）▪Lambda DNA/HindIII Marker, ready-to-use（货号#SM0103）▪Lambda DNA/EcoRI plus HindIII Marker, ready-to-use（货号#SM0193）▪pUC19 DNA/MspI (HpaII) Marker（货号#SM0221）▪pUC19 DNA/MspI (HpaII) Marker, ready-to-use （货号#SM0223）▪GeneRuler 100 bp DNA Ladder （货号#SM0241）▪GeneRuler 100 bp DNA Ladder, ready-to-use（货号#SM0243）▪DNA电泳 |
QQ:1749072012