| Thermo Scientific MboII restriction enzyme recognizes GAAGA(8/7)^ sites and cuts best at 37°C in B buffer. SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as aFastDigest enzymefor rapid DNA digestion.Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.Notes:Assayed using lambda DNA (dam-) (#SD0021). MboI is blocked by overlapping dam methylation. To avoid dam methylation, use a dam-, dcm- strain, such as GM2163. MboI, Bsp143I, and DpnI all recognize the same sequence, but have different methylation sensitivities and cleavage sites. For methylation sensitivity, refer to product specifications.Thermo Scientific MboII限制性内切酶可识别GAAGA（8/7）^位点，并在37°C的B种缓冲液中切割效果最佳。有关此酶及其他限制酶的酶活性，双重消化条件和热灭活条件，请参见限制酶的反应条件表格。 注意：也可以作为FastDigest酶用于快速DNA消化。Thermo Scientific常规限制性内切核酸酶是大量高质量的限制性内切酶，经过优化可在五种缓冲液系统的一种缓冲液中工作。 此外，通用的Tango缓冲液为双消化提供了便利。 在推荐的缓冲液和反应条件下，所有酶均具有100％的活性。 为确保性能稳定，Thermo Scientific限制性内切酶反应缓冲液包含预混合的BSA，可增强许多酶的稳定性并结合DNA制备物中可能存在的污染物。注意：使用lambda DNA（dam-）（＃SD0021）进行分析。 MboI被重叠的dam甲基化所阻断。为避免dam甲基化，请使用dam-，dcm-菌株，例如GM2163。 MboI，Bsp143I和DpnI都识别相同的序列，但具有不同的甲基化敏感性和切割位点。有关甲基化敏感性的信息，请参阅产品说明。 |
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