FastDigest PteI（BssHII、BsePI）/快酶 PteI
货号：FD2134
规格：20 reactions
价格：396
产品类型：内切酶
品牌：Thermo Fisher
| Thermo Scientific FastDigest PteI restriction enzyme recognizes G^CGCGC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: BssHII, BsePI.Thermo Scientific FastDigest PteI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. SeeReaction Conditions for FastDigest Enzymesfor a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such asKlenow Fragment,T4 DNA Ligase, alkaline phosphatases andT4 DNA Polymeraseall have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects. |
| 优点： |
| • 100% activity of all FastDigest enzymes in the universal buffer• 100% buffer compatibility with downstream applications• Complete digestion in 5–15 minutes• Direct loading on gels• No star activity• 176 FastDigest enzymes available |
| 数据： |
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技术参数 产品应用 • Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis
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