Phusion Blood Direct PCR Master Mix/Phusion 血液直接 PCR混合液
货号：F175L,F175S
规格：500 reactions,100 reactions
价格：4698,1192
产品类型：PCR 及DNA聚合酶
品牌：Thermo Fisher
| Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase is resistant to main PCR inhibitors present in blood and retains its activity at blood concentrations of up to 40% in the PCR reaction.This accurate proofreading DNA polymerase makes the Phusion Blood Direct PCR Master Mix an ideal choice even for demanding applications, such as cloning. Phusion Blood Direct PCR Master Mix also allows analysis of blood samples with different preservatives (e.g., heparin, EDTA, citrate), storage conditions and from various species.The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that ofTaq-based polymerases.For optimal results start by accurately calculating your Tm with ourTm calculator. |
优点： |
| - Direct PCR—sample is added directly to PCR reaction, therefore therese is no need for time-consuming and expensive DNA purification steps- Dilution & Storage protocol available—allows tens of PCR reactions from one tiny sample and allows re-testing- Specially engineered Phusion DNA polymerase—extremely short PCR protocol times- Master Mix format with premixed gel loading dye—minimizes possibility of cross-contamination, reduces sample handling and allows direct loading on gel |
数据： |
图1.Extreme robustness with a wide range of amplicon lengths237 bp, 2 kb, and 7.5 kb fragments were amplified directly from human blood (EDTA-treated) with Phusion Blood Direct PCR Master Mix and other vendors' kits. Blood was added directly into reactions and PCR was performed according to suppliers' instructions.M - ZipRuler Express DNA Ladder 2图2.Robust amplification from a wide variety of speciesAmplification of a 237bp DNA fragment directly from whole blood of seven different vertebrates (5%blood in the reaction). + and - denote control reactions图3.Fastest protocol from your sample to a loaded gelComparison of different Blood Direct PCR kits in the market. With Phusion Blood Direct PCR Master mix complete protocol is only 40 min.图4.Amplifies from a variety of blood samplesDNA successfully amplified from human blood and blood from variety of animals.1, 2, 3, 4 — human blood preserved with heparin, citrate, EDTA and stored on cards;5 — mouse; 6 — cat; 7- dog; 8 — cow; 9 — avian; 10 — sheep; 11 — horse; 12 — fish.M — O'GeneRuler Express DNA Ladder+ control reaction with purified DNA- no-template control图5.High yields from blood samples stored on various cardsAmplification of three DNA fragments from human blood collected on various filter papers. Piko Thermal Cycler and UTW reaction vessels were used in PCR. + and - denote control reactions.1 – 903 Card2 – FTA Gene Card3 – FTA Elute Card |
组成成分： |
| ▪5 x 1 mL Phusion Blood Direct PCR Master Mix (2X)▪ 40 µL Universal Control Primer Mix▪ 0.5 mL 50 mM EDTA▪ 1.5 mL 50 mM MgCl2▪ 0.5 mL DMSO▪ 4 x 1.25 mL Water, nuclease-free▪ 50 µg O'GeneRuler Express DNA Ladder (sufficient for 100 applications)Store at -20°C. |
相关产品： |
| ▪DNA 片段标准品▪DNA ladder▪DNA 聚合酶▪PCR▪DNA纯化▪RNA纯化 |
技术参数 产品应用 • Genotyping
• Transgene detection
• Knockout analysis
• Sequencing
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