Taq DNA Polymerase, recombinant (1 U/µL)
货号：EP0404
规格：500 units
价格：226
产品类型：PCR 及DNA聚合酶
品牌：Thermo Fisher
描述Thermo ScientificTaqDNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacteriumThermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition,TaqDNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. RecombinantTaqDNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.Highlights•Thermostable—half life is more than 40 min at 95°C•Generates PCR products with 3'-dA overhangs•Supplied with two buffers—10XTaqBuffer with KCl and 10XTaqBuffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming•Incorporates modified nucleotides(e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)Applications• Routine PCR amplification of DNA fragments up to 5 kb• High throughput PCR• DNA labelingNote• The error rate ofTaqDNA Polymerase in PCR is 2.2 x 10-5errors per nt per cycle. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.• The 10XTaqBuffer without Detergent is recommended for microarray experiments.
QQ:1749072012