RapidOut DNA Removal Kit

货号：K2981

规格：50 preps

价格：687

产品类型：DNA提取

品牌：Thermo Fisher

描述Thermo Scientific RapidOut DNA Removal Kit rapidly and safely removes genomic DNA from total RNA and mRNA preparations. Complete digestion of DNA and safe removal of DNase I from the digestion reaction is ensured without RNA damaging steps, such as heating or organic extraction. First, the RNA sample is treated with recombinant RNase-free DNase I to levels below the limit of detection by routine PCR. DNase I is safely removed subsequently using proprietary DNase Removal Reagent (DRR).DRR efficiently binds DNase I and the complex is collected at the bottom of the tube by centrifugation. The purified RNA is collected as a supernatant. The RNA after RapidOut procedure is free from DNA contamination and free of DNase I. It is ready to use in different applications including end-point or real-time RT-PCR, cloning, microarrays, and Northern blotting.Highlights•Efficient—complete ds and ssDNA digestion and proprietary technology for DNase I removal•Rapid—single step sufficient for complete DNase I removal•Safe—no need for toxic organic extractions or RNA-damaging heating stepsApplicationsMain Applications• RNA isolation and RNA analysis, particularly RT-qPCR and RT-PCR (customers performing expression analysis of low transcription level genes.• Customers performing ds-cDNA synthesis from total RNA preps.Other applications• Elimination of DNA from RNA for microinjections and transfection.• Elimination of DNA from RNA prior microarray analysis.• Elimination of DNA from RNA prior Northern blot analysis.Genomic DNA contamination is reduced below detectable levelsJurkat RNA was purified with Thermo Scientific™ GeneJet™ RNA Purification kit (#K0731/2) and combined with blood gDNA. This mixture was treated with DNase I and the resulting pure RNA was subsequently used in qPCR.Blue curveGreen curve

amplification signal from genomic DNA present in RNA sample prior RapidOut procedure

absence of the signal after qPCR reaction demonstrates absence of gDNA in the RNA sample after RapidOut procedure

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