| 产品详细信息Description: The 6H6 monoclonal antibody reacts with human CD123, the alpha chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.Applications Reported: This 6H6 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 6H6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IL-3Ra (Interleukin-3 receptor subunit alpha) belongs to the type I cytokine receptor family and is a heterodimer with a unique alpha chain paired with the common beta (beta c or CDw131) subunit. IL-3Ra is responsible for the transmission of signal of Interleukin-3. IL-3Ra and IL-3Rb chain heterodimerization is induced by Interleukin-3, which is required for receptor activation but not high affinity binding. IL-3Ra is also reported that IL-3Ra subunits are overexpressed on acute myeloid leukemia(AML) blasts compared with normal hematopoietic cells and are thus potential targets for novel therapeutic agents. |
| 1.Frontiers in immunologyNeutrophils Inhibit Synthesis of Mineralized Extracellular Matrix by Human Bone Marrow-Derived Stromal CellsIn Vitro."Published figure using CD123 monoclonal antibody (Product # 47-1239-42) in Flow Cytometry"2.PloS oneFrailty in old age is associated with decreased interleukin-12/23 production in response to toll-like receptor ligation."Published figure using CD123 monoclonal antibody (Product # 47-1239-42) in Flow Cytometry"3.PLoS pathogensMalaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection."Published figure using CD123 monoclonal antibody (Product # 47-1239-42) in Flow Cytometry4.Journal of clinical pathologyCD56-positive haematological neoplasms of the skin: a multicentre study of the Cutaneous Lymphoma Project Group of the European Organisation for Research and Treatment of Cancer.AuthorsAssaf C,Gellrich S,Whittaker S,Robson A,Cerroni L,Massone C,Kerl H,Rose C,Chott A,Chimenti S,Hallermann C,Petrella T,Wechsler J,Bagot M,Hummel M,Bullani-Kerl K,Bekkenk MW,Kempf W,Meijer CJ,Willemze R,Sterry W5.Nature immunologyEpithelial cells trigger frontline immunoglobulin class switching through a pathway regulated by the inhibitor SLPI.AuthorsXu W,He B,Chiu A,Chadburn A,Shan M,Buldys M,Ding A,Knowles DM,Santini PA,Cerutti A |
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