| The Qubit RNA BR (Broad-Range) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of high-abundance RNA samples. The assay is highly selective for RNA and will not quantitate DNA, protein or free nucleotides. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay. The assay kit is designed to be accurate for initial RNA sample concentrations from 1 ng/µL to 1 µg/µL providing an assay range from 20–1,000 ng. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer.Which product to choose for RNA BR (Broad Range) quantitation?• For 1–20 samples: use this Qubit RNA BR Assay Kit with the Qubit Fluorometer• For 20–2000 samples: use the Quant-iT RNA BR Assay Kit with microplate reader |
Qubit is specific for DNA or RNA.Selectivity of the Qubit assays compared to UV spectroscopy. Triplicate samples containing lambda DNA (10 ng/µL) and varying amounts of ribosomal E. coli RNA (0–100 ng/µl) were assayed using Qubit DNA BR and Qubit RNA BR assays on the Qubit 2.0 Fluorometer according to kit protocols. The same samples were subsequently measured in triplicate using a NanoDrop ND-1000 Spectrophotometer, and single measurements were made using a Perkin Elmer Lambda 35 Spectrophotometer. The concentrations indicated are the concentrations of DNA and RNA in the starting samples, before dilution in the Qubit assay tubes. The red and orange trendlines indicate the actual concentrations of DNA and RNA, respectively, in the starting samples. The actual concentration of nucleic acid was set by diluting pure, concentrated solutions of DNA and RNA to an optical density of 1.0 at 260 nm using a Perkin Elmer Lambda 35 Spectrophotometer. The concentrations of the stock solutions were then calculated and used for all subsequent dilutions. With UV analysis, results for samples containing both DNA and RNA are nondiscriminatory—you cannot distinguish one from the other. |
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