CD279 (PD-1) Monoclonal Antibody (J43), Functional Grade, eBioscience™/CD279 (PD-1) 单克隆 (J43), 功能抗体, eBioscience™

货号：16-9985-85,16-9985-82,16-9985-38,16-9985-025,16-9985-050

规格：500 µg,100 µg,5 mg,25 mg,50 mg

价格：3847,1992,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD279

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：J43

荧光染料：其它

类型:	一抗（功能抗体）	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.Applications Reported: The J43 antibody has been reported for use in flow cytometric analysis. It has also been reported for use in in vitro functional assays.Applications Tested: The J43 antibody has been tested by flow cytometric analysis of mouse Con-A activated spleen cells and mouse PD-1 transfected cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of theimmune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.仅用于科研。不用于诊断过程。

数据

CD279 (PD-1) Antibody (16-9985-82) in FlowStaining of 3-day unstimulated (left) and 3-day ConA-stimulated (right) BALB/c splenocytes with 0.25 µg of Armenian Hamster IgG Isotype Control Functional Grade Purified (Product # 16-4888-81) (open histogram) or 0.25 µg of Anti-Mouse CD279 (PD-1) Functional Grade Purified (filled histogram) followed by Anti-Armenian Hamster IgG Biotin (Product # 13-4113-85) and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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参考文献：

流式1.OncoimmunologyThe stress kinase GCN2 does not mediate suppression of antitumor T cell responses by tryptophan catabolism in experimental melanomas."Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry"2.OncoimmunologyGrowth and metastasis of lung adenocarcinoma is potentiated by BMP4-mediated immunosuppression."Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry"3.Journal of CancerPD-1 Blockade Overcomes Adaptive Immune Resistance in Treatment with Anchored-GM-CSF Bladder Cancer Cells Vaccine."16-9985 was used in Flow cytometry/Cell sorting to investigate whether PD-L1 mediated adaptive resistance could occur in treatment with Anchored-GM-CSF vaccine and whether PD-1 blockade combined with Anchored-GM-CSF vaccine could induce a greater anti-tumour immune response than either immunotherapy alone."AuthorsZhang X,Shi X,Li J,Mo L,Hu Z,Gao J,Wu S,Long Z中和4.Nature communicationsNeuronal IFN-beta-induced PI3K/Akt-FoxA1 signalling is essential for generation of FoxA1+Tregcells."16-9985 was used in Neutralization experiments to investigate the role of IFN in generation of FoxA1(+)Treg cells."AuthorsLiu Y,Marin A,Ejlerskov P,Rasmussen LM,Prinz M,Issazadeh-Navikas S功能检测5.GliaGlial cells suppress postencephalitic CD8+ T lymphocytes through PD-L1."16-9985 was used in Functional assays to demonstrate that microglia and astrocytes control antiviral T-cell responses."AuthorsSchachtele SJ,Hu S,Sheng WS,Mutnal MB,Lokensgard JR6.The Journal of experimental medicineThe programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice.AuthorsAnsari MJ,Salama AD,Chitnis T,Smith RN,Yagita H,Akiba H,Yamazaki T,Azuma M,Iwai H,Khoury SJ,Auchincloss H,Sayegh MH

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