IL-15/IL-15R Complex Monoclonal Antibody (GRW15PLZ), Functional Grade, eBioscience™/IL-15/IL-15R Complex 单克隆 (GRW15PLZ), 功能抗体, eBioscience™

货号： 16-8156-82,16-8156-38,16-8156-025,16-8156-050

规格：500 µg,5 mg,25 mg,50 mg

价格：2564,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：IL-15/IL-15R

物种：小鼠

宿主：大鼠

抗体亚型：IgG1, kappa

克隆号：GRW15PLZ

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	Assay-Dependent（中和）;Assay-Dependent（功能检测）

产品详细信息Description: The monoclonal antibody GRW15PLZ recognizes the mouse IL-15/IL-15 receptor complex. Interleukin-15 (IL-15) is a 14 kDa proinflammatory protein that is has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2receptor, while the alpha subunit is unique to IL-15.Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted when in complex with its unique receptor, IL-15R alpha. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone.The monoclonal antibody GRW15PLZ reacts with and inhibits the bioactivity of IL-15 and the IL-15/IL-15R complex.Applications Reported: The monoclonal antibody GRW15PLZ reacts with and inhibits the bioactivity of IL-15 and the IL-15/IL-15R complex.Applications Tested: The ND50 of GRW15PLZ, as determined by the inhibition of CTLL-2 cell proliferation, is 15-60 ng/mL in the presence of 6 ng/mL recombinant mouse IL-15/IL-15R complex, or 0.4-0.8 µg/mL in the presence of 120 ng/mL recombinant mouse IL-15. Neutralization dose will vary depending on assay method, cytokine concentration, and cell type.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Interleukin-15 (IL-15) is a 14 kDa proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15.Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15R alpha. This chaperoning is required from the ER to Golgi through to secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta/gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages, and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone.仅用于科研。不用于诊断过程。

数据

IL-15/IL-15R Complex Antibody (16-8156-82) in FNNeutralization of Mouse IL-15/IL-15R Complex Recombinant Protein activity as measured by proliferation of CTLL-2 cells.

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参考文献：

流式1.Journal of immunology (Baltimore, Md. : 1950)Tim-3/galectin-9 regulate the homeostasis of hepatic NKT cells in a murine model of nonalcoholic fatty liver disease.AuthorsTang ZH,Liang S,Potter J,Jiang X,Mao HQ,Li Z功能检测2.The Journal of experimental medicineSustained effector function of IL-12/15/18-preactivated NK cells against established tumors."16-8156 was used in Flow cytometry/Cell sorting to reveal how CD4+ T cells have an essential role in sustained anti-tumour activity by NK cells linking adaptive and innate immunity."AuthorsNi J,Miller M,Stojanovic A,Garbi N,Cerwenka A

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