CD117 (c-Kit) Monoclonal Antibody (ACK2), Functional Grade, eBioscience™/CD117 (c-Kit) 单克隆 (ACK2), 功能抗体, eBioscience™

货号：16-1172-81,16-1172-82,16-1172-85,16-1172-38,16-1172-025,16-1172-050

规格：50 µg,100 µg,500 µg,5 mg,25 mg,50 mg

价格：934,1752,3274,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD117 (c-Kit)

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：ACK2

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.125 µg/test（Flow）;Assay-Dependent（功能检测）

产品详细信息Description: The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1^lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.Applications Reported: The ACK2 antibody has been reported for use in flow cytometric analysis. It has also been reported for use in functional assays. (Fluorochrome-conjugated ACK2 is recommended for use in flow cytometric analysis).Applications Tested: The ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息c-Kit, also known as mast/stem cell growth factor receptor (SCFR) or CD117, is a trans membrane type III tyrosine kinase receptor encoded by the c-Kit proto-oncogene, located on chromosome 4 in humans. It is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.仅用于科研。不用于诊断过程。

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参考文献：

流式1.The ProstateLow Testosterone Alters the Activity of Mouse Prostate Stem Cells."Published figure using CD117 (c-Kit) monoclonal antibody (Product # 16-1172-82) in Flow Cytometry"2.The Journal of clinical investigationGATA4-dependent organ-specific endothelial differentiation controls liver development and embryonic hematopoiesis."Published figure using CD117 (c-Kit) monoclonal antibody (Product # 16-1172-82) in Flow Cytometry"IF3.Evidence-based complementary and alternative medicine : eCAMElectroacupuncture at ST36 Increases Bone Marrow-Derived Interstitial Cells of Cajal via the SDF-1/CXCR4 and mSCF/Kit-ETV1 Pathways in the Stomach of Diabetic Mice."Published figure using CD117 (c-Kit) monoclonal antibody (Product # 16-1172-82) in Immunofluorescence"4.Scientific reportsBioengineering functional smooth muscle with spontaneous rhythmic contraction in vitro."Published figure using CD117 (c-Kit) monoclonal antibody (Product # 16-1172-82) in Immunocytochemistry"中和5.Pediatric researchAntagonism of stem cell factor/c-kit signaling attenuates neonatal chronic hypoxia-induced pulmonary vascular remodeling."16-1172 was used in Neutralization experiments to study how SCF/c-kit signaling may potentiate chronic hypoxia-induced vascular remodeling by modulating ERK activation."AuthorsYoung KC,Torres E,Hehre D,Wu S,Suguihara C,Hare JM

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