DyLight 680 Antibody Labeling Kit/DyLight 680抗体标记试剂盒
货号：53056
规格：3 reactions
价格：5921
产品类型：蛋白标记
品牌：Thermo Fisher
| The Thermo Scientific DyLight 680 Antibody Labeling Kit contains an NHS ester-activated derivative of high-performance DyLight 680 used to fluorescently label antibodies and other proteins that are then used as molecular probes for cellular imaging and other fluorescence detection methods. The standard size kit contains all necessary components to perform three separate labeling reactions using 1 mg of IgG or similar quantities of other proteins—the amine-reactive DyLight 680 NHS-ester in convenient single-use vials, as well as purification resin and spin columns for the preparation of ready-to-use conjugate..DyLight 680produces near-infrared (IR) fluorescence that replaces other near-IR dyes, including Cy5.5 dye and Alexa Fluor 680, and is ideal for multi-color applications.The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates. DyLight 680 Amine-Reactive Dye is also available as a stand-alone reagent.DyLight 680 Amine-Reactive Dye is activated with anN-hydroxysuccinimide (NHS) ester moiety to react with exposed N-terminal α-amino groups or the ε-amino groups of lysine residues to form stable amide bonds. Learn more aboutNHS ester chemistry.Typical labeling reactions require the dye to first be dissolved in anhydrous dimethyl formamide (DMF) or another suitable organic solvent before adding a specific molar amount of dye to an amine-free buffer containing the protein to be labeled. However, the high solubility of DyLight Fluors permits protein solutions to be added directly to specific amounts of the labeling reagent. This feature allows DyLight 680 Amine-Reactive Dye to be provided in multiple formats with flexible protocols to achieve efficient degrees of labeling. |
| 特点 |
| •High performance—DyLight 680 fluoresces brighter than Alexa Fluor 680 and Cy5.5 dye•Specific—NHS ester-activated dye labels proteins and other molecules at primary amines (-NH2)•Convenient kit sizes—standard and microscale sizes are offered to match your experimental needs•Optimized procedure—following the standard protocol results in antibodies with excellent dye:protein ratios and recovery rates for optimum activity and fluorescence labeling |
| 应用 |
| • Primary antibody labeling for immunofluorescence microscopy, immunohistochemistry (IHC), Western blotting or ELISA assay• Target protein labeling forin vitroandin vivofluorescent detection strategies |
| 数据 |
| DyLight Antibody Labeling Kits provide outstanding recoveryThe percent recovery for DyLight Antibody Labeling Kits is the average for 16 labeling reactions using three different antibodies. The percent recovery for DyLight Microscale Antibody Labeling Kits is the average for 13 labeling reactions using three different antibodies. Procedure summary for DyLight 350 Antibody Labeling KitsThe protocol is simple and provides efficient labeling, exceptional clean-up and excellent antibody recovery. Immunofluorescence microscopy using DyLight 680 dyeCytokeratin 18 (pseudo-colored yellow) and lamin A (blue) in A549 cells were fluorescently labeled with their respective primary antibodies and DyLight 680-Conjugated Goat Anti-Rabbit or DyLight 405-Conjugated Goat Anti-Mouse Secondary Antibodies, respectively. |
技术参数 组成成分 • 3 vials DyLight NHS Ester• 1 mL tube borate buffer• 5 mL purification resin• 6 spin columns• 12 microcentrifuge collection tubesStore the DyLight NHS Ester at -20°C. Store all other kit components at 4°C.
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