Chemical structure of Phosphine-PEG3-BiotinThis biotinylation reagent is for labeling and detection of azide-tagged molecules via the Staudinger ligation reaction chemistry.
Properties of Phosphine-PEG3-Biotin
Example strategy forin vivometabolic labeling with biotin using Phosphine-PEG3-BiotinWhen bioorthogonal azido-sugar derivatives are supplied to living cells, they become incorporated into glycoprotein structures by endogenous post-translational modification mechanisms. The azide-tagged molecules can then be selectively labeled or conjugated to phosphine-activated molecules, in this case, a derivative of biotin. If the azido sugars were supplied to cells being studied for response to a particular treatment, the biotin affinity-tag could be used to purify and analyze differences in glycosylation resulting from the treatment regime.
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