CD25 Monoclonal Antibody (PC61.5), Functional Grade, eBioscience™/CD25 单克隆 (PC61.5), 功能抗体, eBioscience™

货号：16-0251-85,16-0251-38,16-0251-025,16-0251-050

规格：500 µg,5 mg,25 mg,50 mg

价格：3216,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD25

物种：小鼠

宿主：大鼠

抗体亚型：IgG1, kappa

克隆号：PC61.5

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Fow）;Assay-Dependent（中和）;Assay-Dependent（功能检测）

产品详细信息Description: The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.Applications Reported: The PC61.5 antibody has been reported for use in flow cytometric analysis, and in vitro blocking of mouse CD25 (IL-2R alpha, p55).Applications Tested: The PC61.5 antibody has been tested by flow cytometric analysis of mouse spleen cells and Con A-stimulated splenocyte culture. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.仅用于科研。不用于诊断过程。

数据

CD25 Antibody (16-0251-85) in FlowStaining of 3-day ConA activated C57Bl/6 splenocytes with 0.25 µg of Rat IgG1 kappa Isotype Control Purified (Product # 14-4301-82) (open histogram) or 0.25 µg of Anti-Mouse CD25 Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85). Total viable cells were used for analysis.

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参考文献：

流式1.Experimental and therapeutic medicineAssessment of tumor growth factor-β1 neutralizing antibody in the treatment of allergic rhinitis and asthma."Published figure using CD25 monoclonal antibody (Product # 16-0251-85) in Flow Cytometry"2.Biology openPRDM14 promotes RAG-dependent Notch1 driver mutations in mouse T-ALL."Published figure using CD25 monoclonal antibody (Product # 16-0251-85) in Flow Cytometry"功能检测3.Frontiers in microbiologyOral Administration of Heat-Killed Mycobacterium manresensis Delays Progression toward Active Tuberculosis in C3HeB/FeJ Mice."16-0251 was used in Functional assays to suggest that the use of heat-killed M. manresensis could be a new and promising tool for avoiding active TB induction and as adjunctive to TB treatment."AuthorsCardona P,Marzo-Escartín E,Tapia G,Díaz J,García V,Varela I,Vilaplana C,Cardona PJ

4. Infection and immunity Accumulation of Regulatory T Cells and Chronic Inflammation in the Middle Ear in a Mouse Model of Chronic Otitis Media with Effusion Induced by Combined Eustachian Tube Blockage and Nontypeable Haemophilus influenzae Infection. "16-0251 was used in Functional assays to indicate that regulatory T-cells confer infectious tolerance to Nontypeable Haemophilus influenzae in the middle ear." AuthorsHirano T,Kodama S,Kawano T,Suzuki M 中和5.Immunology and cell biologyPotent anti-tumor responses to immunization with dendritic cells loaded with tumor tissue and an NKT cell ligand."16-0251 was used in Neutralization experiments to investigate the impact of eliciting T-cell help from activated iNKT cells using alpha-galactosylceramide, showing potent anti-tumour response."AuthorsPetersen TR,Sika-Paotonu D,Knight DA,Dickgreber N,Farrand KJ,Ronchese F,Hermans IF

技术参数

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