CD183 (CXCR3) Monoclonal Antibody (CXCR3-173), Functional Grade, eBioscience™/CD183 (CXCR3) 单克隆(CXCR3-173), 功能抗体e, eBioscience™

货号：16-1831-81, 16-1831-85,16-1831-38,16-1831-025,16-1831-050

规格：50 µg,500 µg,5 mg,25 mg,50 mg

价格：1992,3847,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD183 (CXCR3)

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：CXCR3-173

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）；Assay-Dependent（功能检测）

产品详细信息Description: The monoclonal antibody CXCR3-173 recognizes mouse CD183 also known as CXCR3. CD183 is a seven transmembrane G-protein liked chemokine receptor which binds three ligands; CXCL9 (mig), CXCL10 (IP-10)and CXCL11 (ITAC). CD183 as been shown to play a role in CD4 T cell responses to grafts. CXCR3 knockout mice have compromised allograft rejection responses. Expression is found on NK cells, a subset of T lymphocytes and a subset of Tregs as well as preferential expression on Th1-polarizedcells.The antibody CXCR3-173 has been shown to affect chemotaxis in response to ligand. The presence of ligand eliminates staining with the antibody. In vivo addition of the antibody delays cardiac and pancreatic islet allograft rejection.Applications Reported: This CXCR3-173 antibody has been reported for use in flow cytometric analysis.Applications Tested: This CXCR3-173 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed IP10 (interferon-g-inducible 10 kDa protein), Mig (monokine induced by interferon-g) and I-TAC (interferon-inducible T cell a-chemoattractant). IP10, Mig and I-TAC belong to the structural subfamily of CXC chemokines, in which a single amino acid residue separates the first two of four highly conserved Cys residues. Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Inhibition by Bordetella pertussis toxin suggests that heterotrimeric G protein of the Gi-subclass couple to this protein. Signal transduction has not been further analyzed but may include the same enzymes that were identified in the signaling cascade induced by other chemokine receptors. As a consequence of chemokine-induced cellular desensitization (phosphorylation-dependent receptor internalization), cellular responses are typically rapid and short in duration. Cellular responsiveness is restored after dephosphorylation of intracellular receptors and subsequent recycling to the cell surface. This gene is prominently expressed in in vitro cultured effector/memory T cells, and in T cells present in many types of inflamed tissues. In addition, IP10, Mig and I-TAC are commonly produced by local cells in inflammatory lesion, suggesting that this gene and its chemokines participate in the recruitment of inflammatory cells. Therefore, this protein is a target for the development of small molecular weight antagonists, which may be used in the treatment of diverse inflammatory diseases. Multiple transcript variants encoding different isoforms have been found for this gene.仅用于科研。不用于诊断过程。

数据

CD183 (CXCR3) Antibody (16-1831-81) in Flow Staining of C57Bl/6 splenocytes with Anti-Mouse CD8a APC (Product # 17-0081-82) and 0.25 µg of Armenian Hamster IgG Isotype Control Functional Grade Purified (Product # 16-4888-81) (left) or 0.25 µg of Anti-Mouse CD183 (CXCR3) Functional Grade Purified (right) followed by Anti-Armenian Hamster IgG PE (Product # 12-4112-83).Total viable cells were used for analysis.

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参考文献：

中和1.Scientific reportsAntibody-dependent fragmentation is a newly identified mechanism of cell killing in vivo."16-1831 was used in Neutralization experiments to investigate a novel antibody-dependent cell death pathway."AuthorsLiew PX,Kim JH,Lee WY,Kubes P流式2.Immunoendocrinology (Houston, Tex.)PD-1 pathway-mediated regulation of islet-specific CD4+T cell subsets in autoimmune diabetes."16-1831 was used in Flow cytometry/Cell sorting to understand the role of PD-1 in maintaining islet-specific tolerance in diabetes-resistant strains."AuthorsMartinov T,Spanier JA,Pauken KE,Fife BT功能检测3.Critical care (London, England)Therapeutic efficacy of CXCR3 blockade in an experimental model of severe sepsis."16-1831 was used in a Functional assay to evaluate the effects of treatment with anti-CXC chemokine receptor 3 immunoglobulin and antibiotics on outcome, during septic shock caused by cecal ligation puncture."AuthorsHerzig DS,Guo Y,Fang G,Toliver-Kinsky TE,Sherwood ER4.American journal of respiratory and critical care medicineRegulation of lymphocyte trafficking by CXC chemokine receptor 3 during septic shock."16-1831 was used in Functional assays to ascertain the importance of CXC chemokine receptor 3 as a regulator of lymphocyte trafficking during sepsis and determine the contribution of CXCR3-mediated lymphocyte trafficking to the pathogenesis of septic shock."AuthorsHerzig DS,Driver BR,Fang G,Toliver-Kinsky TE,Shute EN,Sherwood ER

技术参数

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