SIGLEC H Monoclonal Antibody (eBio440c), APC
货号：17-0333-82
规格：100μg
价格：2684
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2b, kappa
克隆号：eBio440c
荧光染料：APC
| 抗体类型 | 单抗 | 同型对照： | Rat IgG2b kappa Isotype Control (eB149/10H5), APC |
| 浓度： | 0.2 mg/mL | 用法： | 0.25 μg/test (Flow)； |
| 产品详细信息Description: The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction. The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG).It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.Applications Reported: This eBio440c antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio440c antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser靶标信息Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction. The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG). It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6. |
| 数据 |
| SIGLEC H Antibody (17-0333-82) in FlowC57BL/6 mouse splenocytes were stained with CD45R Monoclonal Antibody, FITC (Product # 11-0452-82) and 0.125 µg of Rat IgG2b kappa Isotype Control, APC (Product # 17-4031-82) (left) or 0.125 µg of Siglec H Monoclonal Antibody, APC (right). Cells in the lymphocyte gate were used for analysis. |
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